In vitro screening of CNS drug candidates

Medium throughput screening on cortical cultures
Various Primary Cell Cultures: Cortex, Hippocampus, Raphe

Typical Neuroprotection Paradigm on primary culture

In vitro screening models available for neuroprotection

• Glutamate toxicity
• Hypoglycemia
• Anoxia

Map2 labeling (fluorescent labeling)

Outcome measures

• Automated quantification of the percentage of Map2 immunopositive surface (i.e. using the Cellomics® Imager)
• Counting the number of neurons
• High throughput analysis by qPCR
• Determination of EC50

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